Alanyl
丙氨酰-tRNA 合成酶蛋白促进两步过程，用 ATP 激活丙氨酸形成 Ala-AMP，并将其转移到 tRNA (Ala) 的受体末端。此外，它还通过其编辑域纠正带错误电荷的 tRNA(Ala)。Alanyl-tRNA synthetase 蛋白, Human (sf9, His) 是重组的 丙氨酰-tRNA 合成酶蛋白，由 Sf9 insect cells 表达，带有 N-His 标签。
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规格 价格 是否有货 数量 5 μg ￥650 In-stock 10 μg ￥1000 In-stock 50 μg ￥2600 In-stock 100 μg ￥3800 In-stock > 100 μg 询价* Please select Quantity before adding items.
生物活性
技术参数
产品性质
产品资料
描述The Alanyl-tRNA synthetase protein facilitates a two-step process, activating alanine with ATP to form Ala-AMP and transferring it to the acceptor end of tRNA(Ala). Additionally, it corrects incorrectly charged tRNA(Ala) through its editing domain. Alanyl-tRNA synthetase Protein, Human (sf9, His) is the recombinant human-derived Alanyl-tRNA synthetase protein, expressed by Sf9 insect cells , with N-His labeled tag.
研究背景丙氨酰-tRNA 合成酶是蛋白质合成中的关键角色，它通过细致的两步过程协调丙氨酸与 tRNA (Ala) 的附着。最初，丙氨酸被 ATP 激活，导致 Ala-AMP 的形成。随后，该活化的丙氨酸被努力转移至 tRNA(Ala) 的受体末端。除了其主要的氨酰化功能外，丙氨酰-tRNA 合成酶还表现出显着的编辑域，使其能够纠正带错误电荷的 tRNA(Ala)。这种双重功能强调了丙氨酰-tRNA 合成酶在维持翻译过程的准确性和保真度方面的关键作用，强调了其在细胞蛋白质合成中的重要性。
生物活性The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
种属Human
表达系统Sf9 insect cells
标签N-10*His
蛋白编号P49588 (M1-N968)
基因 ID16 [NCBI]
蛋白结构N-term
10*His
AARS1 (M1-N968)
Accession # P49588
C-term
蛋白长度Full Length
中文名Alanyl-tRNA synthetase 蛋白
同用名Alanine--tRNA ligase, cytoplasmic; AlaRS; AARS1; AARS
分子量Approximately 109.2 kDa
纯度Greater than 90% as determined by reducing SDS-PAGE.
性状溶液
组分Supplied as a 0.2 μm filtered solution of 20 mM PB, 500 mM NaCl, pH 7.0, 20% Glycerol.
内毒素含量<1 EU/μg, determined by LAL method.
复溶方法N/A.
保存条件 & 期限Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
运输条件Shipping with dry ice.
文件资料Select Batch:
Data Sheet (488 KB) SDS (251 KB)
COA (209 KB)
产品使用指南 (1213 KB)
Help & FAQs
Do most proteins show cross-species activity?Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
复溶计算器
重组蛋白稀释计算器
比活力计算器
The reconstitution calculator equation
Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
The dilution calculator equation
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
The specific activity calculator equation
Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
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