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Functionality and DNA

泰然健康网
2025-08-04 05:03

ABSTRACT Glyphosate (GL) inhibits the aromatic amino acid biosynthesis in plants and is worldwide the most used non-selective herbicide. Less is known about in vivo effects of GL contaminated feedstuffs on the health of dairy cows. The aim of this study was to examine the effects of GL residues in feed at different concentrate feed proportions (CFP) on haematology, immunological and redox parameters and on DNA-damage of blood cells in lactating dairy cows. During a 16-trial, 61 German Holstein cows (207 ± 49 d in milk; mean ± SD) were fed the same ration in week 0. Afterwards, they were assigned to either a group receiving a GL contaminated or a group receiving an uncontaminated total mixed ration (CON). Each group was subdivided into a “low concentrate” group (LC) and a “high concentrate” group (HC) with an energy content of 6.63 MJ NEL and 7.18 MJ NEL/kg dry matter, respectively. The diets were offered for ad libitum consumption. Blood samples were taken at weeks 0, 4, 8, 12 and 16. All blood samples were analysed for white and red blood cell counts. T-cell subpopulations, oxidative burst capability of leukocytes, apoptosis rate, phagocytic activity, activities of superoxide dismutase and glutathione peroxidase, the total non-enzymatic antioxidative capacity, viability and stimulation capacity of peripheral blood mononuclear cells and micronucleus- and comet assay on bovine leukocytes were measured only in week 16. The average individual GL intake of groups CON, GLLC and GLHC was 1.2 µg, 112.6 µg and 132.8 µg per kg body weight and day, respectively. GL contamination did not affect any of the tested parameters whereas CFP and time-influenced leukocytes, granulocytes, red blood cells, haemoglobin, haematocrit, mean corpuscular volume, mean corpuscular haemoglobin and CD4+ T-cells in an interactive manner characterised by a time-dependent increase in HC groups. It can be concluded that GL and GL in combination with different CFP showed no influence on any of the tested endpoints, whereas CFP and time influenced most parameters in an interactive manner.

中文翻译：

不同饲料能量水平下接触草甘膦污染饲料的泌乳牛血细胞的功能和 DNA 损伤特性

摘要草甘膦（GL）抑制植物中芳香族氨基酸的生物合成，是世界范围内使用最广泛的非选择性除草剂。关于 GL 污染的饲料对奶牛健康的体内影响知之甚少。本研究的目的是研究不同精料饲料比例 (CFP) 饲料中 GL 残留物对泌乳奶牛血液学、免疫学和氧化还原参数以及血细胞 DNA 损伤的影响。在一项 16 项试验中，61 头德国荷斯坦奶牛（牛奶中 207 ± 49 天；平均值 ± SD）在第 0 周饲喂相同的日粮。随后，它们被分配到接受 GL 污染的组或接受未污染的组。总混合日粮（CON）。每组又分为“低浓度”组（LC）和“高浓度”组（HC），能量含量分别为6.63 MJ NEL/kg干物质和7.18 MJ NEL/kg干物质。这些饮食可供随意食用。在第 0、4、8、12 和 16 周采集血样。分析所有血样的白细胞和红细胞计数。 T细胞亚群、白细胞氧化爆发能力、凋亡率、吞噬活性、超氧化物歧化酶和谷胱甘肽过氧化物酶活性、总非酶抗氧化能力、外周血单核细胞活力和刺激能力以及牛微核和彗星试验仅在第16周测量白细胞。CON、GLLC和GLHC组的平均个体GL摄入量分别为每公斤体重和每天1.2微克、112.6微克和132.8微克。 GL 污染不影响任何测试参数，而 CFP 和受时间影响的白细胞、粒细胞、红细胞、血红蛋白、红细胞比容、平均红细胞体积、平均红细胞血红蛋白和 CD4+ T 细胞以交互方式影响，其特征是时间依赖性HC组增加。可以得出结论，GL 和 GL 与不同 CFP 的组合对任何测试终点均没有影响，而 CFP 和时间以交互方式影响大多数参数。