茶多酚对60Co γ辐射诱发CHL细胞染色体畸变和微核形成作用的影响
摘要
目的 研究茶多酚（tea polyphenols，TP）对60Coγ辐射诱发中国仓鼠肺成纤维细胞（Chinese hamster fibroblast cell line，CHL）染色体畸变和小鼠骨髓微核形成作用的影响。方法 将CHL细胞（±S9）分为空白对照组、阳性对照组、辐射模型组，TP保护组（分别加TP12.5、25、50 μg/ml）6组，除空白和阳性对照组外均给予2 Gy 60Co γ照射后继续培养24 h，常规制备染色体，观察畸变类型并计算畸变率。50只KM小鼠分为正常对照组、辐射模型组，TP保护组（高、中、低剂量分别于照射后给TP725、145、29 mg/kg灌胃），每组10只，给药后14天给予6 Gy 60Co γ照射，24 h后处死动物，取双侧股骨，行骨髓涂片和姬姆萨染色，计数含微核嗜多染红细胞（polychromatic erythrocyte，PCE）数。结果 中、高剂量组TP（25、50 μg/ml）可显著降低60Co γ诱发的CHL染色体畸变率，S9不影响CHL细胞染色体畸变形式和畸变率。高、中剂量TP保护组（725、145 mg/kg）小鼠PCE微核率与辐照模型组相比较，相差显著（P<0.05）。结论 茶多酚能部分对抗60Co γ辐射诱发的CHL细胞染色体畸变和微核形成，对染色体损伤具有保护作用。
Abstract
Objective To study the effect of tea polyphenols(TP) on chromosomal aberration of Chinese hamster fibroblast cells(CHL) and micronucleus formation induced by radiation.Methods CHL cells (with and without S9)were divided into control group, positive control group, radiation group and TP protective group which were given TP12.5、25、50 μg/ml. Both cells were exposed to 2 Gy 60Co γ rays except control group and positive control group. After cultured in 1640 for 24 hours, cells were harvested for the preparation of chromosome. Subsequently, the ratio and the type of chromosomal aberration were examined. 50 mice were divided into control group, 60Co γ ray radiation group and TP protective group which were given a gavage of TP 725, 145, 29 mg/kg. 10 mice each group. After 14 days the mice were received 6 Gy 60Co γ radiation and were executed after radiation exposure 24 h. By means of bone marrow smear and Giemsa staining, the number of micronucleus cells per 1 000 polychromatic erythrocyte (PCE) was counted.Results The ratios of chromosomal aberration of TP protective group(25、50 μg/ml) were significantly decreased compared with radiation group. S9 has no effect on the type and rate of chromosomal aberration. Compared with radiation group, the micronucleus ratio of TP protective group (725 mg/kg and 145 mg/kg) were significantly decreased(P<0.05).Conclusion TP could partly resist chromosomal aberration of CHL cells and micronucleus formation induced by radiation. TP has protective effects on damage of chromosome.
关键词
茶多酚 /CHL细胞 /染色体 /微核{{custom_keyword}} /
Key words
Tea Polyphenols /Chinese Hamster Fibroblast Cell Line /Chromosome Aberration /Micronucleus{{custom_keyword}} /
李奇慧, 王芳, 王修德, 张惠彬, 顾恰敏, 上官陶, 邹仲敏, 唐木涛.茶多酚对60Co γ辐射诱发CHL细胞染色体畸变和微核形成作用的影响[J]. 中国辐射卫生, 2018, 27(1): 21-24 https://doi.org/10.13491/j.cnki.issn.1004-714x.2018.01.006
LI Qihui, WANG Fang, WANG Xiude, ZHANG Huibin, GU Qiamin, SHANGGUAN Tao, ZOU Zhongmin, TANG Mutao.Effect of tea polyphenols on chromosomal aberration of CHL cells and micronucleus formation induced by radiation[J]. Chinese Journal of Radiological Health, 2018, 27(1): 21-24 https://doi.org/10.13491/j.cnki.issn.1004-714x.2018.01.006
参考文献
[1] 李奇慧,唐木涛,赵勇,等.茶多酚对60Co γ射线诱导L5178Y细胞凋亡及氧化应激的保护作用[J].第三军医大学学报,2014,36(16):1699-1702.
[2] 王硕,黄晓波,许清泉,等.茶多酚在草酸和一水草酸钙诱导HK-2细胞损伤中的保护作用[J].北京大学学报(医学版)2013, 45(4):567-574.
[3] O'Callaghan Yvonne C, Drummond Elaine, O'Gorman Denise M, et al. Antioxidant and pro-apoptotic effects of marine-derived, multi-mineral aquamin supplemented with a pine bark extract, Enzogenol, and a green tea extract, Sunphenon[J]. Journal of medicinal food, 2013, 16(10):920-6.
[4] 李奇慧,朱明学,张黎明,等.硫芥诱导小鼠染色体畸变和微核形成作用的研究[J].第三军医大学学报,2004,26(19):1701-1703.
[5] 王岳飞,梁善珠,张士康,等.茶多酚抗辐射制剂安全毒理学研究[J].茶叶科学,2011,31(5):405-410.
{{custom_fnGroup.title_cn}}
脚注
{{custom_fn.content}}相关知识
辐射威胁：揭示辐射对人体健康和肠道菌群的影响及防护
茶多酚对动物健康有何保护作用
辐射对孕妇的影响
环境污染对健康的影响
茶多酚对人体的主要功效
茶多酚的作用与禁忌
室内电磁辐射污染对人体健康的影响及防护
电磁辐射对人体健康的影响.ppt
电磁辐射对人体健康的影响课件.ppt
体外高频热疗机电磁辐射对健康人的影响及对策
网址: 茶多酚对60Co γ辐射诱发CHL细胞染色体畸变和微核形成作用的影响 https://m.trfsz.com/newsview548120.html
