一月龄婴儿粪便菌群对五种非消化糖的体外发酵作用
LIANG Yu, ZHAO Peng-hao, SHANG Jia-cui, LIU Rui-na, HE Jing, HOU Yu-jia, MENG Xiang-chen. Effect of 1-month-old Infant Fecal Microflora on In Vitro Fermentation of Five Non-digestible Polysaccharides[J]. Science and Technology of Food Industry, 2019, 40(21): 72-77. DOI: 10.13386/j.issn1002-0306.2019.21.012
Citation: LIANG Yu, ZHAO Peng-hao, SHANG Jia-cui, LIU Rui-na, HE Jing, HOU Yu-jia, MENG Xiang-chen. Effect of 1-month-old Infant Fecal Microflora on In Vitro Fermentation of Five Non-digestible Polysaccharides[J]. Science and Technology of Food Industry, 2019, 40(21): 72-77. DOI: 10.13386/j.issn1002-0306.2019.21.012
一月龄婴儿粪便菌群对五种非消化糖的体外发酵作用
东北农业大学乳品科学教育部重点实验室, 黑龙江哈尔滨 150030
详细信息
作者简介:梁玉(1994-),女,硕士研究生,研究方向:食品微生物与生物技术,E-mail:yliang3420@163.com。
通讯作者:孟祥晨(1970-),女,博士,教授,研究方向:食品微生物与生物技术,E-mail:xchmeng@163.com。
中图分类号: TS201.3
计量 文章访问数: 0232 HTML全文浏览量: 036 PDF下载量: 07 出版历程 收稿日期: 2019-02-17 网络出版日期: 2020-11-12 刊出日期: 2019-10-31Effect of 1-month-old Infant Fecal Microflora on In Vitro Fermentation of Five Non-digestible Polysaccharides
Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China
摘要
摘要: 通过体外发酵培养研究一月龄婴儿粪便菌群对低聚果糖、低聚半乳糖、低聚木糖、菊粉、聚葡萄糖五种非消化糖的发酵作用。采用实时荧光定量PCR技术分析培养完成的发酵液主要细菌组成特点,利用高效液相色谱法分析培养完成的发酵液中乳酸及短链脂肪酸含量。qPCR扩增结果表明:低聚果糖能显著促进婴儿粪便中双歧杆菌属、乳杆菌属的生长,抑制梭菌属的增殖(P<0.05),与空白组的菌属含量比值分别为1.12、1.07、0.92;低聚半乳糖可显著抑制肠杆菌的增殖(P<0.05),与空白组的比值为0.98;低聚木糖可显著降低肠球菌属的含量(P<0.05),与空白组的比值为0.97。色谱检测结果显示:婴儿粪便发酵低聚果糖生成的乳酸、乙酸、丁酸均为最高,含量分别为23.00、11.10、1.47 mmol/L,且生成的乳酸及短链脂肪酸总含量也为最高,含量为40.03 mmol/L。低聚木糖组发酵液中丙酸含量最高,含量为5.35 mmol/L。结果表明,综合对比五种非消化糖对一月龄婴儿粪便中益生菌群促进作用及生成有益代谢产物的能力,低聚果糖是最适于促进一月龄婴儿肠道健康的非消化糖。此外,在试图通过非消化糖调节婴儿肠道菌群时,应注意食物因子的选择不同于成人。
关键词: 一月龄 / 婴儿 / 粪便菌群 / 非消化糖 / 体外发酵Abstract: The fermentation effect of 1-month-old infant fecal microflora on five kinds of non-digested polysaccharides:oligofructose,galactooligosaccharide,xylooligosaccharide,inulin and polydextrose,were strdied through in vitro fcrementation. The characteristics of main microorganisms of zymotic fluid after fermentation were analyzed by real-time fluorescent quantitative PCR. The lactic acid and short-chain fatty acids formed after fermentation were analyzed by high performance liquid chromatography. The results of qPCR amplification showed that oligofructose significantly promoted the growth of Bifidobacterium and Lactobacillus and inhibited the proliferation of Clostridium(P<0.05),the ratio of to the blank group was 1.12,1.07,0.92,respectively. Oligosaccharide galactose significantly inhibited the proliferation of Enterobacter(P<0.05),and the ratio to the blank group was 0.98. Xylo-oligosaccharide significantly reduced the content of Enterococcus(P<0.05),and the ratio to the blank group was 0.97. The chromatographic results showed that the lactic acid,acetic acid and butyric acid produced by the fermentation of oligofructose were the highest,the content was 23.00,11.10,1.47 mmol/L,and total content of lactic acid and short chain fatty acid was also the highest,the content was 40.03 mmol/L. The xylo-oligosaccharide fermentation broth had the highest propionic acid content of 5.35 mmol/L. The results showed that oligofructose was the best choice for promoting the health of intestinal flora in infants of one-month-old by comprehensively comparing the ability of five non-digestible sugars to promote the probiotics in the feces of one-month-old infants and to produce beneficial metabolites. When attempting to regulate infant intestinal flora by non-digesting polysaccharides,it should be noted that the choice of food factors is different from that of adults.
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计量 文章访问数: HTML全文浏览量: PDF下载量: 出版历程 收稿日期: 2019-02-17 网络出版日期: 2020-11-12 刊出日期: 2019-10-31目录
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