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机械化学辅助双水相提取绿茶渣中的茶多酚

泰然健康网
2024-12-22 14:57

开发了一种高效且具备选择性提取能力的机械化学辅助双水相提取技术用于提取绿茶渣中的茶多酚。通过单因素以及响应面优化实验优化了实验条件,结果表明,最优工艺条件为硫酸铵质量分数18%、乙醇质量分数35%、液固比65 mL/g、转速468 r/min、提取时间10 min,在此条件下茶多酚的最大产率为50.01±0.26 mg/g,接近传统机械化学法(52.36±0.34 mg/g);同时机械化学辅助双水相提取法所得产物的含固率为46.2%,远高于传统机械化学法(21.6%);此外,机械化学辅助双水相提取法提取的茶多酚体外抗氧化活性能力明显高于传统方法。

Abstract

Tea residue,as a waste residue produced during tea production and processing as well as daily drinking,are rich in tea polyphenols.An efficient mechanochemical-assisted double aqueous-phase system extraction technique with selective extraction capability is developed for the extraction of tea polyphenols from green-tea residues.Experimental conditions are optimized through single factor and response surface optimization experiments.The optimal process conditions are determined as follows:mass fraction of ammonium sulfate is 18%,mass fraction of ethanol is 35%,liquid-to-solid ratio is 65 mL·g-1,milling speed is 468 rpm,and extraction time is 10 min.Under these conditions,the maximum yield of tea polyphenols is 50.01±0.26 mg·g-1,which approaches that by the traditional mechanochemical-assisted extraction (52.36±0.34 mg·g-1).However,the product obtained by mechanochemical-assisted double aqueous-phase extraction contains 46.2% of solid extract,much higher than that obtained by traditional mechanochemical-assisted extraction (21.6%).In addition,the antioxidant capability of tea polyphenols extracted by mechanochemical-assisted double aqueous-phase extraction is significantly higher than that by the traditional method.

关键词

茶渣 /提取 /机械化学 /双水相 /茶多酚{{custom_keyword}} /

Key words

tea residue /extraction /mechanochemical /double aqueous-phase /tea polyphenols{{custom_keyword}} /

周鑫彪, 向兵.机械化学辅助双水相提取绿茶渣中的茶多酚[J]. 现代化工, 2024, 44(S1): 99-104 https://doi.org/10.16606/j.cnki.issn0253-4320.2024.S1.019

ZHOU Xin-biao, XIANG Bing.Mechanochemical-assisted double aqueous-phase system extraction of tea polyphenols from green tea residue[J]. Modern Chemical Industry, 2024, 44(S1): 99-104 https://doi.org/10.16606/j.cnki.issn0253-4320.2024.S1.019

参考文献

[1] Xi J,Xue Y,Xu Y,et al.Artificial neural network modeling and optimization of ultrahigh pressure extraction of green tea polyphenols[J].Food Chemistry,2013,141(1):320-326.
[2] 贡长生.茶多酚的提取和应用研究进展[J].现代化工,1999,19(3):16-18.
[3] Xu X Y,Zhao C N,Cao S Y,et al.Effects and mechanisms of tea for the prevention and management of cancers:An updated review[J].Critical Reviews in Food Science and Nutrition,2020,60(10):1693-1705.
[4] Khan J,Deb P K,Priya S,et al.Dietary flavonoids:Cardioprotective potential with antioxidant effects and their pharmacokinetic,toxicological and therapeutic concerns[J].Molecules,2021,26(13):4021.
[5] Sun S,Zhao Y,Wang L,et al.Ultrasound-assisted extraction of bound phenolic compounds from the residue of Apocynum venetum tea and their antioxidant activities[J].Food Bioscience,2022,47:101646.
[6] Xu J,Xu Z,Zheng W.A review of the antiviral role of green tea catechins[J].Molecules,2017,22(8):1337.
[7] 黄秋森.有机溶剂提取萃取法生产茶多酚工业试验[J].现代化工,2006,26(9):49-51.
[8] Miao S,Wei Y,Chen J,et al.Extraction methods,physiological activities and high value applications of tea residue and its active components:A review[J].Critical Reviews in Food Science and Nutrition,2023,63(23):12150-12168.
[9] Dalmau E,Rossello C,Eim V,et al.Ultrasound-assisted aqueous extraction of biocompounds from orange byproduct:Experimental kinetics and modeling[J].Antioxidants (Basel),2020,9(4):352.
[10] Šeremet D,Joki Ac＇1 S,Aladi Ac＇1 K,et al.Optimization of heat-,microwave-assisted and subcritical water extraction of phenolic compounds from ground ivy (Glechoma hederacea L.) using response surface methodology[J].Journal of Applied Research on Medicinal and Aromatic Plants,2021,25:100346.
[11] Soltani Firouz M,Farahmandi A,Hosseinpour S.Recent advances in ultrasound application as a novel technique in analysis,processing and quality control of fruits,juices and dairy products industries:A review[J].Ultrasonics-Sonochemistry,2019,57:73-88.
[12] Routray W,Orsat V.Microwave-assisted extraction of flavonoids:A review[J].Food and Bioprocess Technology,2011,5(2):409-424.
[13] Wang P,Tang J.Solvent-free mechanochemical extraction of chondroitin sulfate from shark cartilage[J].Chemical Engineering and Process,2009,48(6):1187-1191.
[14] Wang S,Zhang R,Song X,et al.Mechanochemical-assisted extraction of active alkaloids from plant with solid acids[J].ACS Sustainable Chemical Engineering,2019,7(1):197-207.
[15] Wu K,Ju T,Deng Y,et al.Mechanochemical assisted extraction:A novel,efficient,eco-friendly technology[J].Trends in Food Science & Technol,2017,66:166-175.
[16] Dordevic T,Antov M.Ultrasound assisted extraction in aqueous two-phase system for the integrated extraction and separation of antioxidants from wheat chaff[J].Separation and Purification Technology,2017,182(12):52-58.
[17] Chao Y,Shum H.Emerging aqueous two-phase systems:From fundamentals of interfaces to biomedical applications[J].Chemical Society Reviews,2020,49:114-142.
[18] Iqbal M,Tao Y,Xie S,et al.Aqueous two-phase system (ATPS):An overview and advances in its applications[J].Biological Procedures Online,2016,18(1):18.
[19] Huang Y,Wu X,Zhou S,et al.Biphasic extraction of different polysaccharides from Radix Sophorae Tonkinensis by microwave-assisted aqueous two-phase extraction:Process optimization,structural characterization and mechanism exploration[J].Separation and Purification Technology,2018,207(22):187-198.
[20] Wu K,Xi J.Mechanochemical-assisted extraction of platycodin D from the roots of Platycodon grandiflorum with solid alkalis[J].Industrial Crops and Products,2020,145:112026.
[21] Wang L,Qin P,Hu Y.Study on the microwave-assisted extraction of polyphenols from tea[J].Frontiers of Chemical Engineering in China,2010,4(3):307-313.
[22] Baliyan S,Mukherjee R,Priyadarshini A,et al.Determination of antioxidants by DPPH radical scavenging activity and quantitative phytochemical analysis of Ficus religiosa[J].Molecules,2022,27(4):1326.
[23] Talekar S,Patti A,Vijayraghavan R,et al.Rapid,enhanced and eco-friendly recovery of punicalagin from fresh waste pomegranate peels via aqueous ball milling[J].Journal of Cleaner Production,2019,228(10):1238-1247.