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响应面法优化茶多酚生产工艺及其体外抗氧化活性的研究.pdf

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响应面法优化茶多酚生产工艺及其体外抗氧化活性的研究.pdf

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关键词：响应优化茶多酚生产工艺及其体外氧化活性研究资源描述：

中国食晶議加试验研究
响应面法优化茶多酚生产工.艺及其体
外抗氧化活性的研究
陈丽2,蔡利燕2,蒲凌云2,程远欣,田洪
1.河北省天然色素工程技术研究中心,邯郸057250;
2.晨光生物科技集团股份有限公司,邯郸057250)
摘要:[目的]本文以产品多酚含量及多酚得率为指标,采用单因素实验、响应面优化实验,研究了
茶多酚的生产工艺。[方法」在单因素实验的基础上,以产品中多酚含量及多酚得率为指标,响应面优化茶
多酚浓缩液与乙酸乙酯的萃取比例、乙酸乙酯萃取次数、茶多酚浓缩液与璘酸水溶液的比例,对所得茶多酚
进行体外抗氧化活性研究。[结果]茶多酚的最优工业化生产工艺为:乙酸乙酯用量1:1.6(VV),乙酸乙
酯萃取次数2次,磷酸水溶液用量1:1.62(VV),此条件下产品中的多酚含量为99.32%,多酚得率为
80.96%,茶多酚对DPH?的C,值为L.10mg/mL。[结论」该工艺多酚得率高,所得产品多鼢含量较高,
产品有较强的抗氧活性。
关键词:茶多酚;单因素实验;响应面法;抗氧化;多酚含量
中图分类号:TS202.3文献标识码:A文章编号:1006-2513(2012)06-0122-06
Study on tea polyphenols manufacturing techniques by
response surface method and its antioxidant properties in vitro
CHEN Li", CAI Li-yan", PU Ling-yun, CHENG Yuan-xin, TIAN Hong
(I. Engineening Research Center of Natural Pigment in Hebei Province, Handan
057250; 2. Chenguang Biotech Group Co., Itd, Handan 057250
Abstract: [Objective In this paper, manafacturing techniques of es polyphenols were studied by single tactor test
and response surface method. The total tea polyphenols and yield were the indexes. [ Method The effects of ratio of
concerted luton an th l acetate, ethyl acetae extraction time and ratio of concentrated solution and phosphoric
d solution on the polyphenols content and yield were studied. The antioxidation activities of TP in vitro were investi-
gale. Results The results showed that the optimized manufacturing techniques of tea polyphenols were as follows
ratio of concentrated solution and ethyl acetate at 1 1. 60 (/V), ethyl acetate extraction twice and ratio of concen
traed solution and phosphoric acid solution was at 1. 6(/). The oal tea polyphenols was 9. 32% using e ti
mized technology. The yield was 80.96%. The [Cs value of tea polyphenols on DPPH? is 1. 10mg/ml I Conclusion
The manufacturing technique of tea polyphenols is an efficient method with high total tea polyphenols contend and yield
Key words: tea polyphenols; single factor test; response surface method; antioxidant; polyphenols onlenl
茶多酚是( tea polyphenols,简称TP)天然体,约占茶叶干物质总量的15%~36%,其作为
植物茶?中分离提纯的30多种酚类化合物的复合抗氧化剂的活性高于一般非酚类或单酚羟基类抗
收稿日期
30
通讯作者
作者简介:田洪(1965-),男,高级工程师。主要从事天然产物提取分离技术的研究。
192
012年第6期
万方数据